home *** CD-ROM | disk | FTP | other *** search
/ MacFormat 1995 May / macformat-024.iso / Shareware City / Science / GeneConstructionKit demo folder / vectors / pGEM1 < prev    next >
MacBinary  |  1992-03-30  |  8.0 KB  |  [GCKc/GCK ]

open in: MacOS 8.1     |     Win98     |     DOS

view JSON data     |     view as text

This file was processed as: MacBinary (archive/macBinary).

You can browse this item here: pGEM1

ConfidenceProgramDetectionMatch TypeSupport
10% dexvert MacBinary (archive/macBinary) fallback Supported
10% dexvert Jesper Olsen Module (music/jesperOlsen) magic Supported
100% file MacBinary II, Thu Jul 13 18:23:42 1989, modified Sun Mar 29 20:46:50 1992, creator 'GCK ', type 'GCKc', 7542 bytes "pGEM1" , at 0x1df6 480 bytes resource default (weak)
99% file data default
65% TrID TTComp archive compressed (bin-2K) default (weak)
33% TrID MacBinary 2 default (weak)
0% TrID Sybase iAnywhere database files default (weak)
100% lsar MacBinary default


id metadata
keyvalue
macFileType[GCKc]
macFileCreator[GCK ]



hex view
+--------+-------------------------+-------------------------+--------+--------+
|00000000| 00 05 70 47 45 4d 31 00 | 00 00 00 00 00 00 00 00 |..pGEM1.|........|
|00000010| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000020| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000030| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000040| 00 47 43 4b 63 47 43 4b | 20 00 00 00 00 00 00 00 |.GCKcGCK| .......|
|00000050| 00 00 00 00 00 1d 76 00 | 00 01 e0 a0 e2 c6 ee a5 |......v.|........|
|00000060| fc 23 8a 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |.#......|........|
|00000070| 00 00 00 00 00 00 00 00 | 00 00 81 81 59 c2 00 00 |........|....Y...|
|00000080| 00 00 00 03 ff ff 80 01 | 00 00 00 00 00 00 00 00 |........|........|
|00000090| 00 00 00 fd 00 00 01 3d | 00 00 0b 63 00 00 0b 5f |.......=|...c..._|
|000000a0| 47 41 41 54 41 43 41 41 | 47 43 54 54 47 47 47 43 |GAATACAA|GCTTGGGC|
|000000b0| 54 47 43 41 47 47 54 43 | 47 41 43 54 43 54 41 47 |TGCAGGTC|GACTCTAG|
|000000c0| 41 47 47 41 54 43 43 43 | 43 47 47 47 43 47 41 47 |AGGATCCC|CGGGCGAG|
|000000d0| 43 54 43 47 41 41 54 54 | 43 43 47 47 54 43 54 43 |CTCGAATT|CCGGTCTC|
|000000e0| 43 43 54 41 54 41 47 54 | 47 41 47 54 43 47 54 41 |CCTATAGT|GAGTCGTA|
|000000f0| 54 54 41 41 54 54 54 43 | 47 41 54 41 41 47 43 43 |TTAATTTC|GATAAGCC|
|00000100| 41 47 43 54 47 43 41 54 | 54 41 41 54 47 41 41 54 |AGCTGCAT|TAATGAAT|
|00000110| 43 47 47 43 43 41 41 43 | 47 43 47 43 47 47 47 47 |CGGCCAAC|GCGCGGGG|
|00000120| 41 47 41 47 47 43 47 47 | 54 54 54 47 43 47 54 41 |AGAGGCGG|TTTGCGTA|
|00000130| 54 54 47 47 47 43 47 43 | 54 43 54 54 43 43 47 43 |TTGGGCGC|TCTTCCGC|
|00000140| 54 54 43 43 54 43 47 43 | 54 43 41 43 54 47 41 43 |TTCCTCGC|TCACTGAC|
|00000150| 54 43 47 43 54 47 43 47 | 43 54 43 47 47 54 43 47 |TCGCTGCG|CTCGGTCG|
|00000160| 54 54 43 47 47 43 54 47 | 43 47 47 43 47 41 47 43 |TTCGGCTG|CGGCGAGC|
|00000170| 47 47 54 41 54 43 41 47 | 43 54 43 41 43 54 43 41 |GGTATCAG|CTCACTCA|
|00000180| 41 41 47 47 43 47 47 54 | 41 41 54 41 43 47 47 54 |AAGGCGGT|AATACGGT|
|00000190| 54 41 54 43 43 41 43 41 | 47 41 41 54 43 41 47 47 |TATCCACA|GAATCAGG|
|000001a0| 47 47 41 54 41 41 43 47 | 43 41 47 47 41 41 41 47 |GGATAACG|CAGGAAAG|
|000001b0| 41 41 43 41 54 47 54 47 | 41 47 43 41 41 41 41 47 |AACATGTG|AGCAAAAG|
|000001c0| 47 43 43 41 47 43 41 41 | 41 41 47 47 43 43 41 47 |GCCAGCAA|AAGGCCAG|
|000001d0| 47 41 41 43 43 47 54 41 | 41 41 41 41 47 47 43 43 |GAACCGTA|AAAAGGCC|
|000001e0| 47 43 47 54 54 47 43 54 | 47 47 43 47 54 54 54 54 |GCGTTGCT|GGCGTTTT|
|000001f0| 54 43 43 41 54 41 47 47 | 43 54 43 43 47 43 43 43 |TCCATAGG|CTCCGCCC|
|00000200| 43 43 43 54 47 41 43 47 | 41 47 43 41 54 43 41 43 |CCCTGACG|AGCATCAC|
|00000210| 41 41 41 41 41 54 43 47 | 41 43 47 43 54 43 41 41 |AAAAATCG|ACGCTCAA|
|00000220| 47 54 43 41 47 41 47 47 | 54 47 47 43 47 41 41 41 |GTCAGAGG|TGGCGAAA|
|00000230| 43 43 43 47 41 43 41 47 | 47 41 43 54 41 54 41 41 |CCCGACAG|GACTATAA|
|00000240| 41 47 41 54 41 43 43 41 | 47 47 43 47 54 54 54 43 |AGATACCA|GGCGTTTC|
|00000250| 43 43 43 43 54 47 47 41 | 41 47 43 54 43 43 43 54 |CCCCTGGA|AGCTCCCT|
|00000260| 43 47 54 47 43 47 43 54 | 43 54 43 43 54 47 54 54 |CGTGCGCT|CTCCTGTT|
|00000270| 43 43 47 41 43 43 43 54 | 47 43 43 47 43 54 54 41 |CCGACCCT|GCCGCTTA|
|00000280| 43 43 47 47 41 54 41 43 | 43 54 47 54 43 43 47 43 |CCGGATAC|CTGTCCGC|
|00000290| 43 54 54 54 43 54 43 43 | 43 54 54 43 47 47 47 41 |CTTTCTCC|CTTCGGGA|
|000002a0| 41 47 43 47 54 47 47 43 | 47 43 54 54 54 43 54 43 |AGCGTGGC|GCTTTCTC|
|000002b0| 41 41 54 47 43 54 43 47 | 43 47 43 54 47 54 41 47 |AATGCTCG|CGCTGTAG|
|000002c0| 47 54 41 54 43 54 43 41 | 47 54 54 43 47 47 54 47 |GTATCTCA|GTTCGGTG|
|000002d0| 54 41 47 47 54 43 47 54 | 54 43 47 43 54 43 43 41 |TAGGTCGT|TCGCTCCA|
|000002e0| 41 47 43 54 47 47 47 43 | 54 47 54 47 54 47 43 41 |AGCTGGGC|TGTGTGCA|
|000002f0| 43 47 41 41 43 43 43 43 | 43 43 47 54 54 43 41 47 |CGAACCCC|CCGTTCAG|
|00000300| 43 43 43 47 41 43 43 47 | 43 54 47 43 47 43 43 54 |CCCGACCG|CTGCGCCT|
|00000310| 54 41 54 43 43 47 47 54 | 41 41 43 54 41 54 43 47 |TATCCGGT|AACTATCG|
|00000320| 54 43 54 54 47 41 47 54 | 43 43 41 41 43 43 43 47 |TCTTGAGT|CCAACCCG|
|00000330| 47 54 41 41 47 41 43 41 | 43 47 41 43 54 54 41 54 |GTAAGACA|CGACTTAT|
|00000340| 43 47 43 43 41 43 54 47 | 47 43 41 47 43 41 47 43 |CGCCACTG|GCAGCAGC|
|00000350| 43 41 43 54 47 47 54 41 | 41 43 41 47 47 41 54 54 |CACTGGTA|ACAGGATT|
|00000360| 41 47 43 41 47 41 47 43 | 47 41 47 47 54 41 54 47 |AGCAGAGC|GAGGTATG|
|00000370| 54 41 47 47 43 47 47 54 | 47 43 54 41 43 41 47 41 |TAGGCGGT|GCTACAGA|
|00000380| 47 54 54 43 54 54 47 41 | 41 47 54 47 47 54 47 47 |GTTCTTGA|AGTGGTGG|
|00000390| 43 43 54 41 41 43 54 41 | 43 47 47 43 54 41 43 41 |CCTAACTA|CGGCTACA|
|000003a0| 43 54 41 47 41 41 47 47 | 41 43 41 47 54 41 54 54 |CTAGAAGG|ACAGTATT|
|000003b0| 54 47 47 54 41 54 43 54 | 47 43 47 43 54 43 54 47 |TGGTATCT|GCGCTCTG|
|000003c0| 43 54 47 41 41 47 43 43 | 41 47 54 54 41 43 43 54 |CTGAAGCC|AGTTACCT|
|000003d0| 54 43 47 47 41 41 41 41 | 41 47 41 47 54 54 47 47 |TCGGAAAA|AGAGTTGG|
|000003e0| 54 41 47 43 54 43 54 54 | 47 41 54 43 43 47 47 43 |TAGCTCTT|GATCCGGC|
|000003f0| 41 41 41 43 41 41 41 43 | 43 41 43 43 47 43 54 47 |AAACAAAC|CACCGCTG|
|00000400| 47 54 41 47 43 47 47 54 | 47 47 54 54 54 54 54 54 |GTAGCGGT|GGTTTTTT|
|00000410| 54 47 54 54 54 47 43 41 | 41 47 43 41 47 43 41 47 |TGTTTGCA|AGCAGCAG|
|00000420| 41 54 54 41 43 47 43 47 | 43 41 47 41 41 41 41 41 |ATTACGCG|CAGAAAAA|
|00000430| 41 41 47 47 41 54 43 54 | 43 41 41 47 41 41 47 41 |AAGGATCT|CAAGAAGA|
|00000440| 54 43 43 54 54 54 47 41 | 54 43 54 54 54 54 43 54 |TCCTTTGA|TCTTTTCT|
|00000450| 41 43 47 47 47 47 54 43 | 54 47 41 43 47 43 54 43 |ACGGGGTC|TGACGCTC|
|00000460| 41 47 54 47 47 41 41 43 | 47 41 41 41 41 43 54 43 |AGTGGAAC|GAAAACTC|
|00000470| 41 43 47 54 54 41 41 47 | 47 47 41 54 54 54 54 47 |ACGTTAAG|GGATTTTG|
|00000480| 47 54 43 41 54 47 41 47 | 41 54 54 41 54 43 41 41 |GTCATGAG|ATTATCAA|
|00000490| 41 41 41 47 47 41 54 43 | 54 54 43 41 43 43 54 41 |AAAGGATC|TTCACCTA|
|000004a0| 47 41 54 43 43 54 54 54 | 54 41 41 41 54 54 41 41 |GATCCTTT|TAAATTAA|
|000004b0| 41 41 41 54 47 41 41 47 | 54 54 54 54 41 41 41 54 |AAATGAAG|TTTTAAAT|
|000004c0| 43 41 41 54 43 54 41 41 | 41 47 54 41 54 41 54 41 |CAATCTAA|AGTATATA|
|000004d0| 54 47 41 47 54 41 41 41 | 43 54 54 47 47 54 43 54 |TGAGTAAA|CTTGGTCT|
|000004e0| 47 41 43 41 47 54 54 41 | 43 43 41 41 54 47 43 54 |GACAGTTA|CCAATGCT|
|000004f0| 54 41 41 54 43 41 47 54 | 47 41 47 47 43 41 43 43 |TAATCAGT|GAGGCACC|
|00000500| 54 41 54 43 54 43 41 47 | 43 47 41 54 43 54 47 54 |TATCTCAG|CGATCTGT|
|00000510| 43 54 41 54 54 54 43 47 | 54 54 43 41 54 43 43 41 |CTATTTCG|TTCATCCA|
|00000520| 54 41 47 54 54 47 43 43 | 54 47 41 43 54 43 43 43 |TAGTTGCC|TGACTCCC|
|00000530| 43 47 54 43 47 54 47 54 | 41 47 41 54 41 41 43 54 |CGTCGTGT|AGATAACT|
|00000540| 41 43 47 41 54 41 43 47 | 47 47 41 47 47 47 43 54 |ACGATACG|GGAGGGCT|
|00000550| 54 41 43 43 41 54 43 54 | 47 47 43 43 43 43 41 47 |TACCATCT|GGCCCCAG|
|00000560| 54 47 43 54 47 43 41 41 | 54 47 41 54 41 43 43 47 |TGCTGCAA|TGATACCG|
|00000570| 43 47 41 47 41 43 43 43 | 41 43 47 43 54 43 41 43 |CGAGACCC|ACGCTCAC|
|00000580| 43 47 47 43 54 43 43 41 | 47 41 54 54 54 41 54 43 |CGGCTCCA|GATTTATC|
|00000590| 41 47 43 41 41 54 41 41 | 41 43 43 41 47 43 43 41 |AGCAATAA|ACCAGCCA|
|000005a0| 47 43 43 47 47 41 41 47 | 47 47 43 43 47 41 47 43 |GCCGGAAG|GGCCGAGC|
|000005b0| 47 43 41 47 41 41 47 54 | 47 47 54 43 43 54 47 43 |GCAGAAGT|GGTCCTGC|
|000005c0| 41 41 43 54 54 54 41 54 | 43 43 47 43 43 54 43 43 |AACTTTAT|CCGCCTCC|
|000005d0| 41 54 43 43 41 47 54 43 | 54 41 54 54 41 41 54 54 |ATCCAGTC|TATTAATT|
|000005e0| 47 54 54 47 43 43 47 47 | 47 41 41 47 43 54 41 47 |GTTGCCGG|GAAGCTAG|
|000005f0| 41 47 54 41 41 47 54 41 | 47 54 54 43 47 43 43 41 |AGTAAGTA|GTTCGCCA|
|00000600| 47 54 54 41 41 54 41 47 | 54 54 54 47 43 47 43 41 |GTTAATAG|TTTGCGCA|
|00000610| 41 43 47 54 54 47 54 54 | 47 43 43 41 54 54 47 43 |ACGTTGTT|GCCATTGC|
|00000620| 54 41 43 41 47 47 43 41 | 54 43 47 54 47 47 54 47 |TACAGGCA|TCGTGGTG|
|00000630| 54 43 41 43 47 43 54 43 | 47 54 43 47 54 54 54 47 |TCACGCTC|GTCGTTTG|
|00000640| 47 54 41 54 47 47 43 54 | 54 43 41 54 54 43 41 47 |GTATGGCT|TCATTCAG|
|00000650| 43 54 43 43 47 47 54 54 | 43 43 43 41 41 43 47 41 |CTCCGGTT|CCCAACGA|
|00000660| 54 43 41 41 47 47 43 47 | 41 47 54 54 41 43 41 54 |TCAAGGCG|AGTTACAT|
|00000670| 47 41 54 43 43 43 43 43 | 41 54 47 54 54 47 54 47 |GATCCCCC|ATGTTGTG|
|00000680| 43 41 41 41 41 41 41 47 | 43 47 47 54 54 41 47 43 |CAAAAAAG|CGGTTAGC|
|00000690| 54 43 43 54 54 43 47 47 | 54 43 43 54 43 43 47 41 |TCCTTCGG|TCCTCCGA|
|000006a0| 54 43 47 54 54 47 54 43 | 41 47 41 41 47 54 41 41 |TCGTTGTC|AGAAGTAA|
|000006b0| 47 54 54 47 47 43 43 47 | 43 41 47 54 47 54 54 41 |GTTGGCCG|CAGTGTTA|
|000006c0| 54 43 41 43 54 43 41 54 | 47 47 54 54 41 54 47 47 |TCACTCAT|GGTTATGG|
|000006d0| 43 41 47 43 41 43 54 47 | 43 41 54 41 41 54 54 43 |CAGCACTG|CATAATTC|
|000006e0| 54 43 54 54 41 43 54 47 | 54 43 41 54 47 43 43 41 |TCTTACTG|TCATGCCA|
|000006f0| 54 43 43 47 54 41 41 47 | 41 54 47 43 54 54 54 54 |TCCGTAAG|ATGCTTTT|
|00000700| 43 54 47 54 47 41 43 54 | 47 47 54 47 41 47 54 41 |CTGTGACT|GGTGAGTA|
|00000710| 43 54 43 41 41 43 43 41 | 41 47 54 43 41 54 54 43 |CTCAACCA|AGTCATTC|
|00000720| 54 47 41 47 41 41 54 41 | 47 54 47 54 41 54 47 43 |TGAGAATA|GTGTATGC|
|00000730| 47 47 43 47 41 43 43 47 | 41 47 54 54 47 43 54 43 |GGCGACCG|AGTTGCTC|
|00000740| 54 54 47 43 43 43 47 47 | 43 47 54 43 41 41 54 41 |TTGCCCGG|CGTCAATA|
|00000750| 43 47 47 47 41 54 41 41 | 54 41 43 43 47 43 47 43 |CGGGATAA|TACCGCGC|
|00000760| 43 41 43 41 54 41 47 43 | 41 47 41 41 43 54 54 54 |CACATAGC|AGAACTTT|
|00000770| 41 41 41 41 47 54 47 43 | 54 43 41 54 43 41 54 54 |AAAAGTGC|TCATCATT|
|00000780| 47 47 41 41 41 41 43 47 | 54 54 43 54 54 43 47 47 |GGAAAACG|TTCTTCGG|
|00000790| 47 47 43 47 41 41 41 41 | 43 54 43 54 43 41 41 47 |GGCGAAAA|CTCTCAAG|
|000007a0| 47 41 54 43 54 54 41 43 | 43 47 43 54 47 54 54 47 |GATCTTAC|CGCTGTTG|
|000007b0| 41 47 41 54 43 43 41 47 | 54 54 43 47 41 54 47 54 |AGATCCAG|TTCGATGT|
|000007c0| 41 41 43 43 43 41 43 54 | 43 47 54 47 43 41 43 43 |AACCCACT|CGTGCACC|
|000007d0| 43 41 41 43 54 47 41 54 | 43 54 54 43 41 47 43 41 |CAACTGAT|CTTCAGCA|
|000007e0| 54 43 54 54 54 54 41 43 | 54 54 54 43 41 43 43 41 |TCTTTTAC|TTTCACCA|
|000007f0| 47 43 47 54 54 54 43 54 | 47 47 47 54 47 41 47 43 |GCGTTTCT|GGGTGAGC|
|00000800| 41 41 41 41 41 43 41 47 | 47 41 41 47 47 43 41 41 |AAAAACAG|GAAGGCAA|
|00000810| 41 41 54 47 43 43 47 43 | 41 41 41 41 41 41 47 47 |AATGCCGC|AAAAAAGG|
|00000820| 47 41 41 54 41 41 47 47 | 47 43 47 41 43 41 43 47 |GAATAAGG|GCGACACG|
|00000830| 47 41 41 41 54 47 54 54 | 47 41 41 54 41 43 54 43 |GAAATGTT|GAATACTC|
|00000840| 41 54 41 43 54 43 54 54 | 43 43 54 54 54 54 54 43 |ATACTCTT|CCTTTTTC|
|00000850| 41 41 54 41 54 54 41 54 | 54 47 41 41 47 43 41 54 |AATATTAT|TGAAGCAT|
|00000860| 54 54 41 54 43 41 47 47 | 47 54 54 41 54 54 47 54 |TTATCAGG|GTTATTGT|
|00000870| 43 54 43 41 54 47 41 47 | 43 47 47 41 54 41 43 41 |CTCATGAG|CGGATACA|
|00000880| 54 41 54 54 54 47 41 41 | 54 47 54 41 54 54 54 41 |TATTTGAA|TGTATTTA|
|00000890| 47 41 41 41 41 41 54 41 | 41 41 43 41 41 41 54 41 |GAAAAATA|AACAAATA|
|000008a0| 47 47 47 47 54 54 43 43 | 47 43 47 43 41 43 41 54 |GGGGTTCC|GCGCACAT|
|000008b0| 54 54 43 43 43 43 47 41 | 41 41 41 47 54 47 43 43 |TTCCCCGA|AAAGTGCC|
|000008c0| 41 43 43 54 47 41 43 47 | 54 43 54 41 41 47 41 41 |ACCTGACG|TCTAAGAA|
|000008d0| 41 43 43 41 54 54 41 54 | 54 41 54 43 41 54 47 41 |ACCATTAT|TATCATGA|
|000008e0| 43 41 54 54 41 41 43 43 | 54 41 54 41 41 41 41 41 |CATTAACC|TATAAAAA|
|000008f0| 54 41 47 47 43 47 54 41 | 54 43 41 43 47 41 47 47 |TAGGCGTA|TCACGAGG|
|00000900| 43 43 43 54 54 54 43 47 | 54 43 54 54 43 41 41 47 |CCCTTTCG|TCTTCAAG|
|00000910| 43 54 47 43 43 54 43 47 | 43 47 43 47 54 54 54 43 |CTGCCTCG|CGCGTTTC|
|00000920| 47 47 54 47 41 54 47 41 | 43 47 47 54 47 41 41 41 |GGTGATGA|CGGTGAAA|
|00000930| 41 43 43 54 43 54 47 41 | 43 41 43 41 54 47 43 41 |ACCTCTGA|CACATGCA|
|00000940| 47 43 54 43 43 43 47 47 | 41 47 41 43 47 47 54 43 |GCTCCCGG|AGACGGTC|
|00000950| 41 43 41 47 43 54 54 47 | 54 43 54 47 54 41 41 47 |ACAGCTTG|TCTGTAAG|
|00000960| 43 47 47 41 54 47 43 43 | 47 47 47 41 47 43 41 47 |CGGATGCC|GGGAGCAG|
|00000970| 41 43 41 41 47 43 43 43 | 47 54 43 41 47 47 47 43 |ACAAGCCC|GTCAGGGC|
|00000980| 47 43 47 54 43 41 47 43 | 47 47 47 54 47 54 54 47 |GCGTCAGC|GGGTGTTG|
|00000990| 47 43 47 47 47 54 47 54 | 43 47 47 47 47 43 47 43 |GCGGGTGT|CGGGGCGC|
|000009a0| 41 47 43 43 41 54 47 41 | 43 43 43 41 47 54 43 41 |AGCCATGA|CCCAGTCA|
|000009b0| 43 47 54 41 47 43 47 41 | 54 41 47 43 47 47 41 47 |CGTAGCGA|TAGCGGAG|
|000009c0| 54 54 47 47 43 54 54 41 | 41 43 54 41 54 47 43 47 |TTGGCTTA|ACTATGCG|
|000009d0| 47 43 41 54 43 41 47 41 | 47 43 41 47 41 54 54 47 |GCATCAGA|GCAGATTG|
|000009e0| 54 41 43 54 47 41 47 41 | 47 54 47 43 41 43 43 41 |TACTGAGA|GTGCACCA|
|000009f0| 54 41 54 43 47 41 43 47 | 43 54 43 54 43 43 43 54 |TATCGACG|CTCTCCCT|
|00000a00| 54 41 54 47 43 47 41 43 | 54 43 43 54 47 43 41 54 |TATGCGAC|TCCTGCAT|
|00000a10| 54 41 47 47 41 41 47 43 | 41 47 43 43 43 41 47 54 |TAGGAAGC|AGCCCAGT|
|00000a20| 41 47 54 41 47 47 54 54 | 47 41 47 47 43 43 47 54 |AGTAGGTT|GAGGCCGT|
|00000a30| 54 47 41 47 43 41 43 43 | 47 43 43 47 43 43 47 43 |TGAGCACC|GCCGCCGC|
|00000a40| 41 41 47 47 41 41 54 47 | 47 54 47 43 41 54 47 43 |AAGGAATG|GTGCATGC|
|00000a50| 41 41 47 47 41 47 41 54 | 47 47 43 47 43 43 43 41 |AAGGAGAT|GGCGCCCA|
|00000a60| 41 43 41 47 54 43 43 43 | 43 43 47 47 43 43 41 43 |ACAGTCCC|CCGGCCAC|
|00000a70| 47 47 47 47 43 43 54 47 | 43 43 41 43 43 41 54 41 |GGGGCCTG|CCACCATA|
|00000a80| 43 43 43 41 43 47 43 43 | 47 41 41 41 43 41 41 47 |CCCACGCC|GAAACAAG|
|00000a90| 43 47 43 54 43 41 54 47 | 41 47 43 43 43 47 41 41 |CGCTCATG|AGCCCGAA|
|00000aa0| 47 54 47 47 43 47 41 47 | 43 43 43 47 41 54 43 54 |GTGGCGAG|CCCGATCT|
|00000ab0| 54 43 43 43 43 41 54 43 | 47 47 54 47 41 54 47 54 |TCCCCATC|GGTGATGT|
|00000ac0| 43 47 47 43 47 41 54 41 | 54 41 47 47 43 47 43 43 |CGGCGATA|TAGGCGCC|
|00000ad0| 41 47 43 41 41 43 43 47 | 43 41 43 43 54 47 54 47 |AGCAACCG|CACCTGTG|
|00000ae0| 47 43 47 43 43 47 47 54 | 47 41 54 47 43 43 47 47 |GCGCCGGT|GATGCCGG|
|00000af0| 43 43 41 43 47 41 54 47 | 43 47 54 43 43 47 47 43 |CCACGATG|CGTCCGGC|
|00000b00| 47 54 41 47 41 47 47 41 | 54 43 54 47 47 43 54 41 |GTAGAGGA|TCTGGCTA|
|00000b10| 47 43 47 41 54 47 41 43 | 43 43 54 47 43 54 47 41 |GCGATGAC|CCTGCTGA|
|00000b20| 54 54 47 47 54 54 43 47 | 47 54 47 41 43 43 41 54 |TTGGTTCG|GTGACCAT|
|00000b30| 54 54 43 43 47 47 47 47 | 54 47 43 47 47 41 41 43 |TTCCGGGG|TGCGGAAC|
|00000b40| 47 47 43 47 54 54 41 43 | 43 41 47 41 41 41 43 54 |GGCGTTAC|CAGAAACT|
|00000b50| 43 41 47 41 41 47 47 54 | 54 43 47 54 43 43 41 41 |CAGAAGGT|TCGTCCAA|
|00000b60| 43 43 41 41 41 43 43 47 | 41 43 54 43 54 47 41 43 |CCAAACCG|ACTCTGAC|
|00000b70| 47 47 43 41 47 54 54 54 | 41 43 47 41 47 41 47 41 |GGCAGTTT|ACGAGAGA|
|00000b80| 47 41 54 47 41 54 41 47 | 47 47 54 43 54 47 43 54 |GATGATAG|GGTCTGCT|
|00000b90| 54 43 41 47 54 41 41 47 | 43 43 41 47 41 54 47 43 |TCAGTAAG|CCAGATGC|
|00000ba0| 54 41 43 41 43 41 41 54 | 54 41 47 47 43 54 54 47 |TACACAAT|TAGGCTTG|
|00000bb0| 54 41 43 41 54 41 54 54 | 47 54 43 47 54 54 41 47 |TACATATT|GTCGTTAG|
|00000bc0| 41 41 43 47 43 47 47 43 | 54 41 43 41 41 54 54 41 |AACGCGGC|TACAATTA|
|00000bd0| 41 54 41 43 41 54 41 41 | 43 43 54 54 41 54 47 54 |ATACATAA|CCTTATGT|
|00000be0| 41 54 43 41 54 41 43 41 | 43 41 54 41 43 47 41 54 |ATCATACA|CATACGAT|
|00000bf0| 54 54 41 47 47 54 47 41 | 43 41 43 54 41 54 41 00 |TTAGGTGA|CACTATA.|
|00000c00| 00 00 8a 00 00 0b 5f 00 | 03 00 00 00 00 00 00 00 |......_.|........|
|00000c10| 33 00 00 00 04 00 f4 00 | 09 00 00 00 00 d4 00 01 |3.......|........|
|00000c20| e4 cc cc cc cc cc cc cc | cc 00 0b 00 80 00 10 00 |........|........|
|00000c30| 10 00 01 00 00 00 00 00 | 33 00 00 00 39 00 00 00 |........|3...9...|
|00000c40| 04 00 f4 00 09 00 00 00 | 00 d4 00 01 e4 cc cc cc |........|........|
|00000c50| cc cc cc cc cc 00 06 00 | 80 00 10 00 10 00 01 00 |........|........|
|00000c60| 00 00 00 00 39 00 00 0b | 5f 00 00 00 04 00 f4 00 |....9...|_.......|
|00000c70| 09 dd 6b 08 c2 06 a2 01 | 50 ff ff ff ff ff ff ff |..k.....|P.......|
|00000c80| ff 00 06 00 80 00 10 00 | 10 00 01 00 00 00 00 00 |........|........|
|00000c90| 6a 00 00 0b 5f 00 02 00 | 00 00 00 00 00 00 39 00 |j..._...|......9.|
|00000ca0| 1a 00 00 00 0c 00 00 00 | 00 00 01 00 42 00 0c 00 |........|....B...|
|00000cb0| 0c 00 03 00 32 00 09 00 | 00 00 00 00 00 00 58 15 |....2...|......X.|
|00000cc0| e4 00 00 00 00 00 00 00 | 00 00 00 04 48 00 00 07 |........|....H...|
|00000cd0| 5d 00 1a 00 00 00 0c 00 | 58 15 b0 00 01 01 42 00 |].......|X.....B.|
|00000ce0| 08 00 0a 00 03 00 32 00 | 09 02 41 ab 54 ea ff 00 |......2.|..A.T...|
|00000cf0| 58 13 ec 00 58 14 0c 00 | 00 00 00 12 70 6f 6c 79 |X...X...|....poly|
|00000d00| 63 6c 6f 6e 69 6e 67 20 | 72 65 67 69 6f 6e 15 41 |cloning |region.A|
|00000d10| 6d 70 69 63 69 6c 6c 69 | 6e 20 72 65 73 69 73 74 |mpicilli|n resist|
|00000d20| 61 6e 63 65 00 00 00 10 | a7 2d 6c 61 63 74 61 6d |ance....|.-lactam|
|00000d30| 61 73 65 20 67 65 6e 65 | 00 00 01 64 00 00 0b 5f |ase gene|...d..._|
|00000d40| 00 07 00 00 00 06 00 00 | 00 0c 00 01 00 05 00 00 |........|........|
|00000d50| 00 00 00 0a 00 00 00 03 | 00 35 00 09 00 00 00 00 |........|.5......|
|00000d60| 00 00 00 58 13 f8 00 58 | 16 98 01 00 00 00 00 0d |...X...X|........|
|00000d70| ff eb fe 5b 00 00 00 0f | 00 00 00 15 00 05 00 01 |...[....|........|
|00000d80| 00 00 00 00 00 0a 00 00 | 00 03 00 65 00 09 00 00 |........|...e....|
|00000d90| 00 00 00 00 00 58 15 94 | 00 00 00 00 01 00 00 00 |.....X..|........|
|00000da0| 00 0e ff ef fe 9e 00 00 | 00 15 00 00 00 1b 00 01 |........|........|
|00000db0| 00 05 00 00 00 00 00 0a | 00 00 00 03 00 00 00 09 |........|........|
|00000dc0| 00 00 00 00 00 00 00 58 | 18 b4 00 00 00 00 01 00 |.......X|........|
|00000dd0| 00 00 00 0f ff f1 ff 2f | 00 00 00 21 00 00 00 27 |......./|...!...'|
|00000de0| 00 01 00 05 00 00 00 00 | 00 0c 00 00 00 03 00 00 |........|........|
|00000df0| 00 09 00 00 00 00 00 00 | 00 58 15 c4 00 58 15 74 |........|.X...X.t|
|00000e00| 01 00 00 00 00 14 ff f1 | ff d2 00 00 00 26 00 00 |........|.....&..|
|00000e10| 00 2c 00 03 00 03 00 00 | 00 00 00 0a 00 00 00 03 |.,......|........|
|00000e20| 00 00 00 09 00 00 00 00 | 00 00 00 58 15 84 00 58 |........|...X...X|
|00000e30| 18 bc 01 00 00 00 00 11 | ff f1 00 8b 00 00 00 2d |........|.......-|
|00000e40| 00 00 00 33 00 05 00 01 | 00 00 00 00 00 0a 00 00 |...3....|........|
|00000e50| 00 03 00 00 00 09 00 00 | 00 00 00 00 00 58 14 10 |........|.....X..|
|00000e60| 00 58 15 a8 01 00 00 00 | 00 10 ff ee 00 ed 00 00 |.X......|........|
|00000e70| 00 33 00 00 00 39 00 01 | 00 05 00 00 00 00 00 0c |.3...9..|........|
|00000e80| 00 00 00 03 00 ff 00 09 | 00 00 00 00 00 00 00 58 |........|.......X|
|00000e90| 17 04 00 58 16 0c 01 00 | 00 00 00 13 ff e9 01 28 |...X....|.......(|
|00000ea0| 07 48 69 6e 64 49 49 49 | 00 00 00 1b 49 73 6f 73 |.HindIII|....Isos|
|00000eb0| 63 68 69 7a 6f 6d 65 72 | 20 77 69 74 68 20 45 63 |chizomer| with Ec|
|00000ec0| 6f 20 56 49 49 49 2e 04 | 50 73 74 49 04 53 61 6c |o VIII..|PstI.Sal|
|00000ed0| 49 05 42 61 6d 48 49 00 | 00 00 18 49 73 6f 73 63 |I.BamHI.|...Isosc|
|00000ee0| 68 69 7a 6f 6d 65 72 20 | 77 69 74 68 20 42 73 74 |hizomer |with Bst|
|00000ef0| 20 49 2e 04 53 6d 61 49 | 00 00 00 1f 49 73 6f 73 | I..SmaI|....Isos|
|00000f00| 63 68 69 7a 6f 6d 65 72 | 20 77 69 74 68 20 58 63 |chizomer| with Xc|
|00000f10| 79 20 49 2c 20 58 6d 61 | 20 49 2e 04 53 73 74 49 |y I, Xma| I..SstI|
|00000f20| 00 00 00 18 49 73 6f 73 | 63 68 69 7a 6f 6d 65 72 |....Isos|chizomer|
|00000f30| 20 77 69 74 68 20 53 61 | 63 20 49 2e 05 45 63 6f | with Sa|c I..Eco|
|00000f40| 52 49 00 00 00 18 49 73 | 6f 73 63 68 69 7a 6f 6d |RI....Is|oschizom|
|00000f50| 65 72 20 77 69 74 68 20 | 52 73 72 20 49 2e 00 00 |er with |Rsr I...|
|00000f60| 00 10 00 00 0b 5f 00 01 | 00 00 00 00 00 00 0b 5f |....._..|......._|
|00000f70| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000f80| 00 00 00 00 44 41 01 30 | 10 ff 0a 3c 00 04 66 28 |....DA.0|...<..f(|
|00000f90| 70 08 b0 6e ff fc 6d 06 | 3e 3c 07 07 60 1a 70 0d |p..n..m.|><..`.p.|
|00000fa0| b0 6e ff fc 00 00 00 00 | 00 00 00 00 70 0f b0 6e |.n......|....p..n|
|00000fb0| ff fc 66 04 3e 3c 07 05 | 30 07 04 40 00 69 67 00 |..f.><..|0..@.ig.|
|00000fc0| 01 26 53 40 67 00 01 d6 | 59 40 67 00 01 e8 04 40 |.&S@g...|Y@g....@|
|00000fd0| 01 ee 67 00 11 20 00 00 | 00 00 00 00 00 00 67 00 |..g.. ..|......g.|
|00000fe0| 12 84 55 40 67 00 12 ec | 53 40 67 00 14 2c 57 40 |..U@g...|S@g..,W@|
|00000ff0| 67 00 11 96 53 40 67 00 | 14 08 04 40 02 50 67 00 |g...S@g.|...@.Pg.|
|00001000| 10 c6 53 40 67 00 10 c0 | 04 40 00 c2 67 00 0f ee |..S@g...|.@..g...|
|00001010| 53 40 67 00 10 84 53 40 | 67 00 10 7e 04 40 00 62 |S@g...S@|g..~.@.b|
|00001020| 67 00 0e 80 53 40 67 00 | 0d 2c 53 40 67 00 0f 1a |g...S@g.|.,S@g...|
|00001030| 04 40 00 61 67 00 01 90 | 04 40 00 c5 67 00 03 7c |.@.ag...|.@..g..||
|00001040| 53 40 67 00 02 ca 53 40 | 67 00 01 ac 53 40 67 00 |S@g...S@|g...S@g.|
|00001050| 06 82 04 40 00 64 6b 00 | 13 da 04 40 00 59 6f 00 |...@.dk.|...@.Yo.|
|00001060| 07 96 04 40 00 0b 6b 00 | 13 ca 04 40 00 59 6f 00 |...@..k.|...@.Yo.|
|00001070| 08 9c 04 40 00 09 67 00 | 08 94 55 40 67 00 04 cc |...@..g.|..U@g...|
|00001080| 04 40 00 60 67 00 04 a2 | 53 40 67 00 04 3c 53 40 |.@.`g...|S@g..<S@|
|00001090| 67 00 04 b8 55 40 67 00 | 04 00 04 40 00 65 6b 00 |g...U@g.|...@.ek.|
|000010a0| 13 92 04 40 00 50 6f 00 | 09 aa 04 40 00 11 67 00 |...@.Po.|...@..g.|
|000010b0| 09 a2 57 40 67 00 0a 10 | 53 40 67 00 0b b0 53 40 |..W@g...|S@g...S@|
|000010c0| 67 00 0a 54 53 40 67 00 | 0a 4e 53 40 67 00 0a 48 |g..TS@g.|.NS@g..H|
|000010d0| 53 40 67 00 0a 42 57 40 | 67 00 0a 3c 04 40 1a 24 |S@g..BW@|g..<.@.$|
|000010e0| 67 5c 60 00 13 4e 42 67 | 48 6e ff ea 48 6e ff da |g\`..NBg|Hn..Hn..|
|000010f0| 2f 0b 4e ad 4c 6a 10 1f | 67 40 2f 0b 4e ad 2e 62 |/.N.Lj..|g@/.N..b|
|00001100| 2f 0b 4e ad 2e 6a 48 6e | ff d4 48 6d 3a 94 3f 3c |/.N..jHn|..Hm:.?<|
|00001110| 00 90 4e ad 02 42 2f 2e | ff d4 4e ad 00 7a 3f 07 |..N..B/.|..N..z?.|
|00001120| 48 6e ff ea 48 6e ff da | 42 67 2f 0b 2f 2e ff d4 |Hn..Hn..|Bg/./...|
|00001130| 4e ba dd 1a 2d 6e ff d4 | 00 0e 60 00 13 04 42 67 |N...-n..|..`...Bg|
|00001140| 48 6e ff d9 48 6e ff ea | 48 6e ff da 2f 0b 4e ad |Hn..Hn..|Hn../.N.|
|00001150| 4c 7a 10 1f 67 42 2f 0b | 4e ad 2e 62 2f 0b 4e ad |Lz..gB/.|N..b/.N.|
|00001160| 2e 6a 48 6e ff d4 48 6d | 3a 94 3f 3c 00 90 4e ad |.jHn..Hm|:.?<..N.|
|00001170| 02 42 2f 2e ff d4 4e ad | 00 7a 3f 07 48 6e ff ea |.B/...N.|.z?.Hn..|
|00001180| 48 6e ff da 1f 2e ff d9 | 2f 0b 2f 2e ff d4 4e ba |Hn......|/./...N.|
|00001190| dc bc 2d 6e ff d4 00 0e | 60 00 12 a6 42 a7 42 40 |..-n....|`...B.B@|
|000011a0| 10 2e ff ff 3f 00 2f 0b | 4e ba fb 5e 2d 5f 00 0e |....?./.|N..^-_..|
|000011b0| 60 00 12 8e 42 40 10 2e | ff ff 3f 00 2f 0b 4e ad |`...B@..|..?./.N.|
|000011c0| 2e 8a 60 00 12 7c 48 6e | ff 64 48 6d 3a 84 3f 3c |..`..|Hn|.dHm:.?<|
|000011d0| 01 38 4e ad 02 42 2f 2e | ff 64 4e ad 00 7a 3f 2e |.8N..B/.|.dN..z?.|
|000011e0| ff fc 2f 0b 2f 2e ff 64 | 05 70 47 45 4d 31 00 00 |.././..d|.pGEM1..|
|000011f0| 0a 6e 54 68 69 73 20 66 | 69 6c 65 20 77 61 73 20 |.nThis f|ile was |
|00001200| 64 6f 77 6e 6c 6f 61 64 | 65 64 20 66 72 6f 6d 20 |download|ed from |
|00001210| 74 68 65 20 31 32 2f 38 | 37 20 56 65 63 42 61 73 |the 12/8|7 VecBas|
|00001220| 65 20 62 79 20 4b 65 69 | 74 68 20 41 20 4a 6f 68 |e by Kei|th A Joh|
|00001230| 6e 73 6f 6e 20 6f 6e 20 | 44 65 63 2e 20 31 34 2c |nson on |Dec. 14,|
|00001240| 20 31 39 38 39 2e 0d 4d | 6f 64 69 66 69 65 64 20 | 1989..M|odified |
|00001250| 62 79 20 42 6f 62 20 47 | 72 6f 73 73 2c 20 31 32 |by Bob G|ross, 12|
|00001260| 2f 32 36 2f 38 39 2e 0d | 0d 0d 45 4e 54 52 59 20 |/26/89..|..ENTRY |
|00001270| 50 47 45 4d 31 20 20 20 | 20 20 20 20 20 20 20 20 |PGEM1 | |
|00001280| 20 20 20 20 20 20 20 20 | 20 23 54 59 50 45 20 44 | | #TYPE D|
|00001290| 4e 41 20 43 49 52 43 55 | 4c 41 52 0d 54 49 54 4c |NA CIRCU|LAR.TITL|
|000012a0| 45 20 70 47 45 4d 31 20 | 2d 20 43 6c 6f 6e 69 6e |E pGEM1 |- Clonin|
|000012b0| 67 20 56 65 63 74 6f 72 | 20 47 65 6d 69 6e 69 20 |g Vector| Gemini |
|000012c0| 31 20 66 72 6f 6d 20 50 | 72 6f 6d 65 67 61 20 42 |1 from P|romega B|
|000012d0| 69 6f 74 65 63 0d 44 41 | 54 45 20 20 20 20 20 20 |iotec.DA|TE |
|000012e0| 32 37 2d 4d 41 52 2d 31 | 39 38 36 0d 20 20 20 23 |27-MAR-1|986. #|
|000012f0| 73 65 71 75 65 6e 63 65 | 20 20 31 31 2d 44 45 43 |sequence| 11-DEC|
|00001300| 2d 31 39 38 36 0d 20 20 | 20 23 73 65 71 75 65 6e |-1986. | #sequen|
|00001310| 63 65 20 20 31 36 2d 44 | 45 43 2d 31 39 38 36 0d |ce 16-D|EC-1986.|
|00001320| 41 43 43 45 53 53 49 4f | 4e 20 56 42 30 30 33 36 |ACCESSIO|N VB0036|
|00001330| 0d 53 4f 55 52 43 45 20 | 20 20 20 61 72 74 69 66 |.SOURCE | artif|
|00001340| 69 63 69 61 6c 0d 52 45 | 46 45 52 45 4e 43 45 0d |icial.RE|FERENCE.|
|00001350| 20 20 20 23 6e 75 6d 62 | 65 72 20 31 0d 20 20 20 | #numb|er 1. |
|00001360| 23 63 69 74 61 74 69 6f | 6e 20 20 50 72 6f 6d 65 |#citatio|n Prome|
|00001370| 67 61 20 74 65 63 68 6e | 69 63 61 6c 20 42 75 6c |ga techn|ical Bul|
|00001380| 6c 65 74 69 6e 0d 20 20 | 20 23 63 6f 6d 6d 65 6e |letin. | #commen|
|00001390| 74 20 20 20 54 68 65 20 | 73 65 71 75 65 6e 63 65 |t The |sequence|
|000013a0| 20 77 61 73 20 72 65 76 | 69 73 65 64 20 62 79 20 | was rev|ised by |
|000013b0| 50 72 6f 6d 65 67 61 20 | 42 69 6f 74 65 63 20 4a |Promega |Biotec J|
|000013c0| 55 4c 2d 31 39 38 36 0d | 20 20 20 20 20 20 20 20 |UL-1986.| |
|000013d0| 20 20 20 20 20 20 50 72 | 6f 6d 65 67 61 20 61 6c | Pr|omega al|
|000013e0| 73 6f 20 63 6f 6e 66 69 | 72 6d 65 64 20 74 68 65 |so confi|rmed the|
|000013f0| 20 72 65 76 69 73 69 6f | 6e 20 6f 66 20 44 45 43 | revisio|n of DEC|
|00001400| 2d 31 39 38 36 0d 20 20 | 20 23 63 6f 6d 6d 65 6e |-1986. | #commen|
|00001410| 74 20 20 63 6f 70 79 72 | 69 67 68 74 20 6f 66 20 |t copyr|ight of |
|00001420| 74 68 65 20 73 65 71 75 | 65 6e 63 65 20 62 79 20 |the sequ|ence by |
|00001430| 50 72 6f 6d 65 67 61 0d | 43 4f 4d 4d 45 4e 54 0d |Promega.|COMMENT.|
|00001440| 20 20 20 45 6e 74 65 72 | 65 64 20 62 79 20 57 69 | Enter|ed by Wi|
|00001450| 6c 6c 69 61 6d 20 47 69 | 6c 62 65 72 74 2c 20 57 |lliam Gi|lbert, W|
|00001460| 68 69 74 61 6b 65 72 20 | 43 6f 6c 6c 65 67 65 2c |hitaker |College,|
|00001470| 20 4d 49 54 0d 20 20 20 | 20 20 20 20 61 6e 64 20 | MIT. | and |
|00001480| 62 79 20 4a 2e 20 53 6f | 70 70 61 2c 20 61 6e 64 |by J. So|ppa, and|
|00001490| 20 46 2e 20 50 66 65 69 | 66 66 65 72 2c 20 4d 50 | F. Pfei|ffer, MP|
|000014a0| 49 2c 20 4d 61 72 74 69 | 6e 73 72 69 65 64 0d 20 |I, Marti|nsried. |
|000014b0| 20 20 52 65 76 69 73 65 | 64 20 31 31 2d 44 45 43 | Revise|d 11-DEC|
|000014c0| 2d 31 39 38 36 20 62 79 | 20 46 2e 20 50 66 65 69 |-1986 by| F. Pfei|
|000014d0| 66 66 65 72 20 61 63 63 | 6f 72 64 69 6e 67 20 74 |ffer acc|ording t|
|000014e0| 6f 20 50 72 6f 6d 65 67 | 61 0d 20 20 20 52 65 76 |o Promeg|a. Rev|
|000014f0| 69 73 65 64 20 31 36 2d | 44 45 43 2d 31 39 38 36 |ised 16-|DEC-1986|
|00001500| 20 62 79 20 46 2e 20 50 | 66 65 69 66 66 65 72 3a | by F. P|feiffer:|
|00001510| 0d 20 20 20 35 33 30 2f | 31 20 22 41 54 22 20 74 |. 530/|1 "AT" t|
|00001520| 6f 20 22 54 41 22 20 74 | 6f 20 6d 61 74 63 68 20 |o "TA" t|o match |
|00001530| 72 65 76 69 73 65 64 20 | 73 65 71 75 65 6e 63 65 |revised |sequence|
|00001540| 20 6f 66 20 50 42 52 33 | 32 32 0d 43 4f 4d 4d 45 | of PBR3|22.COMME|
|00001550| 4e 54 0d 20 20 20 54 68 | 65 20 70 47 45 4d 31 20 |NT. Th|e pGEM1 |
|00001560| 76 65 63 74 6f 72 20 63 | 6f 6e 74 61 69 6e 73 20 |vector c|ontains |
|00001570| 62 6f 74 68 20 74 68 65 | 20 54 37 20 61 6e 64 20 |both the| T7 and |
|00001580| 53 50 36 20 52 4e 41 20 | 70 6f 6c 79 6d 65 72 61 |SP6 RNA |polymera|
|00001590| 73 65 20 70 72 6f 6d 6f | 74 65 72 73 0d 20 20 20 |se promo|ters. |
|000015a0| 73 65 70 65 72 61 74 65 | 64 20 62 79 20 61 20 73 |seperate|d by a s|
|000015b0| 68 6f 72 74 20 70 69 65 | 63 65 20 6f 66 20 44 4e |hort pie|ce of DN|
|000015c0| 41 20 63 6f 6e 74 61 69 | 6e 69 6e 67 20 31 31 20 |A contai|ning 11 |
|000015d0| 75 6e 69 71 75 65 20 72 | 65 73 74 72 69 63 74 69 |unique r|estricti|
|000015e0| 6f 6e 20 73 69 74 65 73 | 2e 0d 20 20 20 49 74 20 |on sites|.. It |
|000015f0| 77 61 73 20 63 6f 6e 73 | 74 72 75 63 74 65 64 20 |was cons|tructed |
|00001600| 66 72 6f 6d 20 70 53 50 | 36 34 20 61 6e 64 20 61 |from pSP|64 and a|
|00001610| 20 62 61 63 74 65 72 69 | 6f 70 68 61 67 65 20 54 | bacteri|ophage T|
|00001620| 37 20 70 72 6f 6d 6f 74 | 65 72 2e 0d 20 20 20 44 |7 promot|er.. D|
|00001630| 75 72 69 6e 67 20 63 6f | 6e 73 74 72 75 63 74 69 |uring co|nstructi|
|00001640| 6f 6e 20 6f 66 20 74 68 | 65 20 53 50 36 20 76 65 |on of th|e SP6 ve|
|00001650| 63 74 6f 72 73 2c 20 74 | 68 65 20 6d 61 6a 6f 72 |ctors, t|he major|
|00001660| 69 74 79 20 6f 66 20 74 | 68 65 20 6c 61 63 5a 20 |ity of t|he lacZ |
|00001670| 67 65 6e 65 0d 20 20 20 | 77 61 73 20 64 65 6c 65 |gene. |was dele|
|00001680| 74 65 64 2e 20 20 54 68 | 65 72 65 66 6f 72 65 2c |ted. Th|erefore,|
|00001690| 20 74 68 65 20 58 2d 67 | 61 6c 20 63 6f 6c 6f 72 | the X-g|al color|
|000016a0| 20 73 63 72 65 65 6e 69 | 6e 67 20 6d 65 74 68 6f | screeni|ng metho|
|000016b0| 64 20 66 6f 72 20 74 68 | 65 0d 20 20 20 69 64 65 |d for th|e. ide|
|000016c0| 6e 74 69 66 69 63 61 74 | 69 6f 6e 20 6f 66 20 72 |ntificat|ion of r|
|000016d0| 65 63 6f 6d 62 69 6e 61 | 6e 74 73 20 69 73 20 6e |ecombina|nts is n|
|000016e0| 6f 74 20 70 6f 73 73 69 | 62 6c 65 20 77 69 74 68 |ot possi|ble with|
|000016f0| 20 74 68 69 73 20 76 65 | 63 74 6f 72 2e 0d 4b 45 | this ve|ctor..KE|
|00001700| 59 57 4f 52 44 53 0d 43 | 52 4f 53 53 52 45 46 45 |YWORDS.C|ROSSREFE|
|00001710| 52 45 4e 43 45 0d 20 20 | 20 23 70 61 72 65 6e 74 |RENCE. | #parent|
|00001720| 0d 20 20 20 20 20 56 65 | 63 42 61 73 65 28 33 29 |. Ve|cBase(3)|
|00001730| 3a 70 53 50 36 34 0d 20 | 20 20 23 70 61 72 65 6e |:pSP64. | #paren|
|00001740| 74 0d 20 20 20 20 20 56 | 65 63 42 61 73 65 28 33 |t. V|ecBase(3|
|00001750| 29 3a 70 55 43 31 32 2c | 20 56 65 63 53 6f 75 72 |):pUC12,| VecSour|
|00001760| 63 65 28 33 29 3a 62 47 | 61 6c 31 32 2c 0d 20 20 |ce(3):bG|al12,. |
|00001770| 20 20 20 56 65 63 53 6f | 75 72 63 65 28 33 29 3a | VecSo|urce(3):|
|00001780| 50 72 6f 6d 53 50 36 2c | 20 56 65 63 53 6f 75 72 |PromSP6,| VecSour|
|00001790| 63 65 28 33 29 3a 50 72 | 6f 6d 54 37 2c 0d 20 20 |ce(3):Pr|omT7,. |
|000017a0| 20 20 20 56 65 63 42 61 | 73 65 28 33 29 3a 70 42 | VecBa|se(3):pB|
|000017b0| 52 33 32 32 0d 20 20 20 | 23 62 72 6f 74 68 65 72 |R322. |#brother|
|000017c0| 0d 20 20 20 20 20 56 65 | 63 42 61 73 65 28 33 29 |. Ve|cBase(3)|
|000017d0| 3a 70 47 45 4d 32 0d 20 | 20 20 23 6f 66 66 73 70 |:pGEM2. | #offsp|
|000017e0| 72 69 6e 67 0d 20 20 20 | 20 20 56 65 63 42 61 73 |ring. | VecBas|
|000017f0| 65 28 33 29 3a 70 47 45 | 4d 33 0d 50 41 52 45 4e |e(3):pGE|M3.PAREN|
|00001800| 54 0d 20 20 20 46 65 61 | 74 75 72 65 73 20 6f 66 |T. Fea|tures of|
|00001810| 20 70 47 45 4d 31 20 28 | 32 38 36 35 20 62 70 29 | pGEM1 (|2865 bp)|
|00001820| 0d 20 20 20 20 20 72 65 | 73 69 64 75 65 20 20 20 |. re|sidue |
|00001830| 20 20 73 6f 75 72 63 65 | 0d 20 20 20 20 20 20 37 | source|. 7|
|00001840| 2d 20 20 35 37 20 20 20 | 20 35 31 2d 20 20 20 31 |- 57 | 51- 1|
|00001850| 20 28 63 29 20 4d 31 33 | 6d 70 31 30 2f 70 55 43 | (c) M13|mp10/pUC|
|00001860| 31 32 2d 70 6f 6c 79 6c | 69 6e 6b 65 72 0d 20 20 |12-polyl|inker. |
|00001870| 20 20 20 36 34 2d 20 20 | 38 33 20 20 20 20 32 30 | 64- |83 20|
|00001880| 2d 20 20 20 31 20 28 63 | 29 20 54 37 20 70 72 6f |- 1 (c|) T7 pro|
|00001890| 6d 6f 74 65 72 0d 20 20 | 20 20 20 39 34 2d 20 31 |moter. | 94- 1|
|000018a0| 35 32 20 20 20 20 20 35 | 39 2d 20 20 31 20 28 63 |52 5|9- 1 (c|
|000018b0| 29 20 70 55 43 31 32 0d | 20 20 20 20 31 35 33 2d |) pUC12.| 153-|
|000018c0| 32 33 34 31 20 20 32 36 | 38 30 2d 20 34 39 32 20 |2341 26|80- 492 |
|000018d0| 28 63 29 20 70 55 43 31 | 32 0d 20 20 20 32 33 34 |(c) pUC1|2. 234|
|000018e0| 32 2d 32 36 32 30 20 20 | 20 36 35 34 2d 20 33 37 |2-2620 | 654- 37|
|000018f0| 36 20 28 63 29 20 70 42 | 52 33 32 32 0d 20 20 20 |6 (c) pB|R322. |
|00001900| 32 36 32 31 2d 32 38 36 | 35 20 20 78 78 78 78 2d |2621-286|5 xxxx-|
|00001910| 78 78 78 78 20 20 20 20 | 20 70 68 61 67 65 20 53 |xxxx | phage S|
|00001920| 50 36 0d 20 20 20 32 38 | 34 37 2d 32 38 36 35 20 |P6. 28|47-2865 |
|00001930| 20 20 20 20 31 2d 20 20 | 31 39 20 20 20 20 20 53 | 1- |19 S|
|00001940| 50 36 20 70 72 6f 6d 6f | 74 65 72 0d 20 20 20 43 |P6 promo|ter. C|
|00001950| 6f 6e 66 6c 69 63 74 20 | 28 63 66 6c 29 20 61 6e |onflict |(cfl) an|
|00001960| 64 20 4d 75 74 61 74 69 | 6f 6e 73 20 28 6d 75 74 |d Mutati|ons (mut|
|00001970| 29 3a 20 6e 6f 6e 65 0d | 50 41 52 45 4e 54 0d 20 |): none.|PARENT. |
|00001980| 20 20 46 65 61 74 75 72 | 65 73 20 6f 66 20 70 47 | Featur|es of pG|
|00001990| 45 4d 31 20 28 32 38 36 | 35 20 62 70 29 0d 20 20 |EM1 (286|5 bp). |
|000019a0| 20 20 20 72 65 73 69 64 | 75 65 20 20 20 20 20 73 | resid|ue s|
|000019b0| 6f 75 72 63 65 0d 20 20 | 20 20 20 20 37 2d 20 20 |ource. | 7- |
|000019c0| 35 37 20 20 20 20 35 31 | 2d 20 20 20 31 20 28 63 |57 51|- 1 (c|
|000019d0| 29 20 4d 31 33 6d 70 31 | 30 2f 70 55 43 31 32 2d |) M13mp1|0/pUC12-|
|000019e0| 70 6f 6c 79 6c 69 6e 6b | 65 72 0d 20 20 20 20 20 |polylink|er. |
|000019f0| 36 34 2d 20 20 38 33 20 | 20 20 20 32 30 2d 20 20 |64- 83 | 20- |
|00001a00| 20 31 20 28 63 29 20 54 | 37 20 70 72 6f 6d 6f 74 | 1 (c) T|7 promot|
|00001a10| 65 72 0d 20 20 20 20 20 | 39 34 2d 32 31 35 34 20 |er. |94-2154 |
|00001a20| 20 20 36 32 30 2d 32 36 | 38 30 20 20 20 20 20 70 | 620-26|80 p|
|00001a30| 55 43 31 32 63 0d 20 20 | 20 32 31 35 35 2d 32 33 |UC12c. | 2155-23|
|00001a40| 34 31 20 20 20 20 20 31 | 2d 20 31 38 37 20 20 20 |41 1|- 187 |
|00001a50| 20 20 70 55 43 31 32 63 | 0d 20 20 20 32 33 34 32 | pUC12c|. 2342|
|00001a60| 2d 32 36 32 30 20 20 20 | 36 35 34 2d 20 33 37 36 |-2620 |654- 376|
|00001a70| 20 28 63 29 20 70 42 52 | 33 32 32 0d 20 20 20 32 | (c) pBR|322. 2|
|00001a80| 36 32 31 2d 32 38 36 35 | 20 20 78 78 78 78 2d 78 |621-2865| xxxx-x|
|00001a90| 78 78 78 20 20 20 20 20 | 70 68 61 67 65 20 53 50 |xxx |phage SP|
|00001aa0| 36 0d 20 20 20 32 38 34 | 37 2d 32 38 36 35 20 20 |6. 284|7-2865 |
|00001ab0| 20 20 20 31 2d 20 20 31 | 39 20 20 20 20 20 53 50 | 1- 1|9 SP|
|00001ac0| 36 20 70 72 6f 6d 6f 74 | 65 72 0d 20 20 20 43 6f |6 promot|er. Co|
|00001ad0| 6e 66 6c 69 63 74 20 28 | 63 66 6c 29 20 61 6e 64 |nflict (|cfl) and|
|00001ae0| 20 4d 75 74 61 74 69 6f | 6e 73 20 28 6d 75 74 29 | Mutatio|ns (mut)|
|00001af0| 3a 20 6e 6f 6e 65 0d 46 | 45 41 54 55 52 45 0d 20 |: none.F|EATURE. |
|00001b00| 20 20 20 20 20 20 20 20 | 20 20 31 20 20 20 20 20 | | 1 |
|00001b10| 20 20 20 20 20 20 73 74 | 61 72 74 20 6f 66 20 53 | st|art of S|
|00001b20| 50 36 2d 52 4e 41 20 73 | 79 6e 74 68 65 73 69 73 |P6-RNA s|ynthesis|
|00001b30| 0d 20 20 20 20 20 20 20 | 20 20 20 36 36 20 20 20 |. | 66 |
|00001b40| 20 20 20 20 28 63 29 20 | 73 74 61 72 74 20 6f 66 | (c) |start of|
|00001b50| 20 54 37 2d 52 4e 41 20 | 73 79 6e 74 68 65 73 69 | T7-RNA |synthesi|
|00001b60| 73 0d 20 20 20 31 30 39 | 37 2d 31 38 38 35 20 37 |s. 109|7-1885 7|
|00001b70| 38 39 2d 31 20 28 63 29 | 20 41 70 2d 52 3b 20 62 |89-1 (c)| Ap-R; b|
|00001b80| 2d 6c 61 63 74 61 6d 61 | 73 65 0d 50 4f 4c 59 4c |-lactama|se.POLYL|
|00001b90| 49 4e 4b 45 52 20 20 45 | 63 6f 52 49 2d 53 61 63 |INKER E|coRI-Sac|
|00001ba0| 49 2d 53 6d 61 49 2d 42 | 61 6d 48 49 2d 58 62 61 |I-SmaI-B|amHI-Xba|
|00001bb0| 49 2d 53 61 6c 49 2d 50 | 73 74 49 2d 48 69 6e 64 |I-SalI-P|stI-Hind|
|00001bc0| 49 49 49 0d 53 45 4c 45 | 43 54 49 4f 4e 20 23 72 |III.SELE|CTION #r|
|00001bd0| 65 73 69 73 74 61 6e 63 | 65 20 20 41 70 0d 53 55 |esistanc|e Ap.SU|
|00001be0| 4d 4d 41 52 59 20 20 70 | 47 45 4d 31 20 20 20 20 |MMARY p|GEM1 |
|00001bf0| 23 6c 65 6e 67 74 68 20 | 32 38 36 35 20 20 20 23 |#length |2865 #|
|00001c00| 63 68 65 63 6b 73 75 6d | 20 37 35 32 30 0d 53 45 |checksum| 7520.SE|
|00001c10| 51 55 45 4e 43 45 0d 0d | 20 56 65 63 62 61 73 65 |QUENCE..| Vecbase|
|00001c20| 3a 50 67 65 6d 31 20 20 | 4c 65 6e 67 74 68 3a 20 |:Pgem1 |Length: |
|00001c30| 32 38 36 35 20 20 44 65 | 63 65 6d 62 65 72 20 31 |2865 De|cember 1|
|00001c40| 31 2c 20 31 39 38 39 20 | 20 31 30 3a 30 34 20 20 |1, 1989 | 10:04 |
|00001c50| 43 68 65 63 6b 3a 20 37 | 35 32 30 20 20 2e 2e 80 |Check: 7|520 ...|
|00001c60| 00 00 00 00 00 00 00 00 | 00 00 00 01 01 01 01 00 |........|........|
|00001c70| 00 01 00 01 00 00 00 10 | 00 10 01 e4 cc cc cc cc |........|........|
|00001c80| cc cc cc cc dd 6b 08 c2 | 06 a2 00 01 01 00 00 08 |.....k..|........|
|00001c90| 00 0a 00 03 00 00 00 09 | 02 41 ab 54 ea ff 00 03 |........|.A.T....|
|00001ca0| 00 00 00 09 00 0a 00 00 | 00 00 00 00 00 04 00 90 |........|........|
|00001cb0| 00 09 00 00 00 00 00 00 | 01 00 3f 80 00 00 00 00 |........|..?.....|
|00001cc0| 00 e1 00 00 01 19 00 00 | 00 fd 00 00 01 3d 00 01 |........|.....=..|
|00001cd0| 00 6d 00 09 00 00 00 00 | 00 00 00 73 00 80 00 00 |.m......|...s....|
|00001ce0| 00 00 06 31 38 32 2e 34 | 30 ad 3f b2 2d 46 00 1c |...182.4|0.?.-F..|
|00001cf0| 60 04 2d 46 00 1c 4c df | 1c c0 4e 5e 20 5f de fc |`.-F..L.|..N^ _..|
|00001d00| 00 14 4e d0 00 00 00 5e | 40 80 e3 7a 40 80 65 44 |..N....^|@..z@.eD|
|00001d10| 00 00 6d 3c 00 00 4c a2 | 00 01 71 00 00 00 00 10 |..m<..L.|..q.....|
|00001d20| 02 64 00 5e 09 40 40 81 | 2d 64 00 6e 00 01 00 6c |.d.^.@@.|-d.n...l|
|00001d30| 70 1a 00 6d f0 ac 40 81 | 27 a8 40 81 27 6e 00 6e |p..m..@.|'.@.'n.n|
|00001d40| 00 01 00 6c 70 1a 00 5e | 09 40 40 81 2d 64 00 6e |...lp..^|.@@.-d.n|
|00001d50| 00 01 00 6c 70 1a 00 6d | f0 ac 00 6d 40 81 27 a8 |...lp..m|...m@.'.|
|00001d60| 40 81 27 6e 00 6e 00 01 | 00 6c 70 1a 00 6d f0 ac |@.'n.n..|.lp..m..|
|00001d70| 00 6d e7 0a 00 6d 6f 6a | 00 5e 85 e0 00 6d f7 30 |.m...moj|.^...m.0|
|00001d80| 00 5e 4c a2 00 6d 6f fa | 00 5e 85 e0 00 5e 7e 78 |.^L..mo.|.^...^~x|
|00001d90| 00 00 f7 30 00 00 e3 22 | 40 80 65 44 00 5e 40 80 |...0..."|@.eD.^@.|
|00001da0| e3 7a 40 80 65 44 00 00 | 6d 60 00 00 4c a2 00 6e |.z@.eD..|m`..L..n|
|00001db0| 00 01 00 6c 70 1a 00 6e | 00 01 00 6c 70 1a 00 6d |...lp..n|...lp..m|
|00001dc0| 6f fa 00 6d 00 6d f7 30 | 00 6d f7 30 00 6d 00 01 |o..m.m.0|.m.0.m..|
|00001dd0| d3 56 00 5e 00 6d e5 9e | 00 5e 46 ba 00 00 01 26 |.V.^.m..|.^F....&|
|00001de0| 00 6e 00 00 00 00 00 00 | 00 20 00 00 00 20 00 04 |.n......|. ... ..|
|00001df0| 00 0c 00 00 00 0c 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00001e00| 00 00 01 00 00 00 01 9a | 00 00 00 9a 00 00 00 46 |........|.......F|
|00001e10| 47 41 41 54 47 41 41 54 | 43 41 43 43 47 41 54 41 |GAATGAAT|CACCGATA|
|00001e20| 43 47 43 47 41 47 43 47 | 41 41 43 47 54 47 41 41 |CGCGAGCG|AACGTGAA|
|00001e30| 05 70 47 45 4d 31 02 00 | 00 00 47 43 4b 63 47 43 |.pGEM1..|..GCKcGC|
|00001e40| 4b 20 00 00 00 00 00 00 | 00 00 00 00 15 24 00 00 |K ......|.....$..|
|00001e50| 00 00 47 43 4b 63 47 43 | 4b 20 00 00 00 00 00 00 |..GCKcGC|K ......|
|00001e60| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00001e70| 00 00 ab 83 7a 1f 00 00 | 1d 76 00 00 01 e0 41 41 |....z...|.v....AA|
|00001e80| 41 43 47 43 47 47 41 41 | 47 54 43 41 47 43 47 43 |ACGCGGAA|GTCAGCGC|
|00001e90| 43 43 54 47 43 41 43 43 | 41 54 54 41 54 47 54 54 |CCTGCACC|ATTATGTT|
|00001ea0| 43 43 47 47 41 54 43 54 | 47 43 41 54 43 47 43 41 |CCGGATCT|GCATCGCA|
|00001eb0| 47 47 41 54 47 43 54 47 | 43 54 47 47 43 54 41 43 |GGATGCTG|CTGGCTAC|
|00001ec0| 43 43 54 47 54 47 47 41 | 41 43 41 43 43 54 41 43 |CCTGTGGA|ACACCTAC|
|00001ed0| 41 54 43 54 47 54 41 54 | 54 41 41 43 47 41 41 47 |ATCTGTAT|TAACGAAG|
|00001ee0| 43 47 43 54 47 47 43 41 | 54 54 47 41 43 43 43 54 |CGCTGGCA|TTGACCCT|
|00001ef0| 47 41 47 54 47 41 54 54 | 54 54 54 43 54 43 54 47 |GAGTGATT|TTTCTCTG|
|00001f00| 00 00 00 78 00 03 00 00 | 00 48 00 50 00 00 00 00 |...x....|.H.P....|
|00001f10| 02 f0 02 80 ff dc ff ec | 02 f4 02 94 01 02 05 28 |........|.......(|
|00001f20| 03 fc 00 01 00 00 00 48 | 00 50 00 00 00 00 02 f0 |.......H|.P......|
|00001f30| 02 80 00 50 00 20 02 80 | 0c 80 00 18 00 01 01 01 |...P. ..|........|
|00001f40| 00 00 00 01 27 0f 00 01 | 01 01 00 00 00 00 00 00 |....'...|........|
|00001f50| 00 00 00 00 00 00 00 00 | 00 00 00 01 00 00 00 00 |........|........|
|00001f60| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00001f70| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 1a |........|........|
|00001f80| 01 24 00 00 00 29 00 00 | 00 03 00 00 01 18 00 00 |.$...)..|........|
|00001f90| 01 4c 00 00 00 00 00 00 | 00 00 00 00 01 00 00 00 |.L......|........|
|00001fa0| 01 9a 00 00 00 9a 00 00 | 00 46 00 66 fc 5c 0b 06 |........|.F.f.\..|
|00001fb0| 00 00 00 1c 00 46 00 01 | 50 52 4e 54 00 00 00 12 |.....F..|PRNT....|
|00001fc0| 73 74 61 74 00 00 00 1e | 00 01 ff ff 00 00 00 00 |stat....|........|
|00001fd0| 00 00 00 00 5b f8 ff ff | 00 00 00 7c 00 67 67 8c |....[...|...|.gg.|
|00001fe0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00001ff0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
+--------+-------------------------+-------------------------+--------+--------+